Biosafety > Biosafety Manual > Standard Microbiological Practices > Containment
- All procedures must be performed in a manner that minimizes the creation of aerosols. These activities include centrifugation (use secondary containers as pictured), vortex mixing (stopper the tubes), blending (use safety blender), sonication,
grinding, opening containers of infectious materials, inoculating
culture flasks, inoculating animals, harvesting infectious materials
from cultures or animals, weighing or reconstituting toxic powders,
- Use a biological safety cabinet for handling infectious materials or materials requiring protection from contamination. Locate biological safety cabinets in low-traffic areas: minimize activities that disrupt airflow in or around cabinet. Use a fume hood for chemicals and toxic materials. Mixed hazards (i.e. infectious agent and toxic chemicals) need individual evaluation for selecting the proper protective equipment.
Contact the Department of Environmental Health and Safety.
- Use liquid disinfectant traps and in-line vacuum filters on all vacuum lines. This protects the vacuum system from contamination and minimizes the risk to those who service and repair the system.