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Home > Biosafety > Biosafety Manual > Select Agents > Procedures for Labs Working with Federally Regulated Select Agents

Procedures for Labs Working with Federally Regulated Select Agents

Select Agents and toxin quantities (per principal investigator), exceeding those shown in the following tables, are subject to federal select agent registration and regulations. Follow the University of Minnesota procedures outlined below to assure federal compliance.

HHS Toxins Amount
Abrin 100 mg
Botulinum neurotoxins 0.5 mg
Short, paralytic alpha conotoxin containing the following amino acid sequence X1CCX2PACGX3X4X5X6CX7)1 100 mg
Diacetoxyscirpenol (DAS) 1000 mg
Ricin 100 mg
Saxitoxin 100 mg
Staphylococcal Enterotoxins (Subtypes A, B, C, D, and E) 5 mg
T-2 toxin 1000 mg
Tetrodotoxin 100 mg

1 C = Cysteine residues are all present as disulfides, with the 1st and 3rd Cysteine, and the 2nd and 4th Cysteine forming specific disulfide bridges; The consensus sequence includes known toxins α-MI and α-GI (shown above) as well as α-GIA, Ac1.1a, α-CnIA, α-CnIB; X1 = any amino acid(s) or Des-X; X2 = Asparagine or Histidine; P = Proline;  A = Alanine;  G = Glycine; X3 = Arginine or Lysine;  X4 = Asparagine, Histidine, Lysine, Arginine, Tyrosine, Phenylalanine or Tryptophan; X5 = Tyrosine, Phenylalanine, or Tryptophan;  X6 = Serine, Threonine, Glutamate, Aspartate, Glutamine, or Asparagine;  X7 = Any amino acid(s) or Des X and; “Des X” = “an amino acid does not have to be present at this position.”  For example if a peptide sequence were XCCHPA then the related peptide CCHPA would be designated as Des-X.

Procedure to Apply for Select Agent Use

Notify the biosafety officer in Environmental Health & Safety (DEHS) 612-626-6002 if you wish to receive a select agent. All transfers of select agents require approval by both The Center for Disease Control (CDC) and the University of Minnesota's select agent program Responsible Official. Shipping and receiving of all select agents must be done through DEHS.

In addition, you must obtain approval from the Institutional Biosafety Committee (IBC) before receiving and/or using select agents at the University. Click here to learn more about applying for IBC review and approval.

Procedures for Approved Select Agent Use

Possession of select agents requires regular communication with DEHS. The procedures below are organized around the timing or frequency with which they occur.

Notify Immediately

Contact DEHS 626-6002 during regular business hours or after hours call 911 and ask for a hazardous materials response for any of the following situations:

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Notify As Soon As Possible

Contact DEHS 626-6002 for:

Monthly

Quarterly

DEHS will audit labs to:

Annual

Continuous

View or download the following documents:
Select Agent Inventory Record of Changes to Long-term Storage
Select Agent Use or Storage Area Access Log – Authorized Individuals
Select Agent Use or Storage Area Access Log – Unauthorized Individuals
Approved Access Individual Signature Log

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