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Biosafety Manual

Table of Contents

1. Accidental Exposure/Emergency Response
1. General Emergency Procedures
2. Biohazards and Toxin Decontamination & Spill Clean-up
3. Biological Decontamination & Spill Clean-up Plan Template
4. Needlesticks and Other Infectious Exposure Procedures
5. Reporting Workers' Compensation Related Injuries
6. Reporting Incidents to the IBC
    
2. Autoclave Safety and Effectiveness
1. Autoclaving Biological Waste
2. Tips to Assure Effective Sterilization by Autoclave
3. Operator Maintenance
4. Autoclave Testing Log
    
3. Biological Safety Cabinets
   1. Definition of Terms
   2. Types (Classes) of Biological Safety Cabinets
   3. Biological Safety Cabinet Placement
   4. Certification
   5. Use of Open Flames
   6. UV Lights
   7. Vacuum Lines
   8. Safe & Effective Work Practices
   9. Spill Cleanup
       
4. Bloodborne Pathogen/Human Infectious Agents
1. Bloodborne Pathogen Standard
2. Bloodborne Pathogen Training (in-person)
3. Bloodborne Pathogen Training (online)
4. University of Minnesota Exposure Control Plan
5. OSHA Bloodborne Pathogen Fact Sheets
6. Needlestick and Other Infectious Exposure Procedures
7. OSHA's Revised Bloodborne Pathogen Standard 2001 PPT INCL Safety Engineered Sharps
8. 24 minute video: Workplace Precautions Against Bloodborne Pathogens
9. OSHA Standards: Bloodborne Pathogens and Needlestick Prevention
10. Biohazards and Toxin Decontamination & Spill Clean-up
11. Working with Human / Other Primate Cells, Tissues, or Human Derived Products
     
5. Certificates
1. Certificate for ATCC ordering
2. Certificate of Environmental Compliance for DOD Application
3. Certificate of Environmental Compliance for DOE Application
    
6. Compliance Requirements for Working with Biohazardous Material

 
7. Decontamination and Disinfectants
1. Decontamination
2. Biological Decontamination & Spill Clean-up Plan Template
3. Biohazards and Toxin Decontamination & Spill Clean-up
4. Preparing Equipment for Service, Transfer, or Disposal
    
8. Fact Sheets: Safe Laboratory Practices
    
9. Infectious Agents
1. Biosafety Level 2 Procedures
2. IBC Infectious Agents Form
    
10. Prions Research Procedures
     
11. Recombinant DNA
1. Fact Sheet for Working with Recombinant DNA Material
2. IBC Recombinant DNA Form
3. NIH Guidelines for Research Involving Recombinant DNA Molecules
4. NIH/OBA Risk Assessment
    
12. Risk Assessment
1. Biological Risk Assessment in BMBL 5th edition
2. Risk Assessment Process
3. Self Assessment Form
4. Introduction to Risk Assessment
    
13. Select Agents
     
14. Sharps Usage
1. Sharps Safety
2. Sharps Safety Fact Sheet
3. OSHA's Revised Bloodborne Pathogen Standard 2001 PPT INCL Safety Engineered Sharps
    
15. Shipping, Transfer, or Disposal of Biological Materials
1. Shipping
• Guidelines for Shipping/Receiving Biological Materials
• Infectious Substance Classification Flow Chart
• Checklist for Diagnostic and Clinical Specimens
• Training:Shipping Hazardous and Infectious Materials
• Register Online for Shipping Hazardous and Infectious Materials Training Class
• Hazardous Material Shipping Program
• Shipping Exempt Patient Specimens
• Shipping Genetically Modified Organisms (GMOs)
• Shipper's Declaration of Dangerous Goods
• DOT training: Transporting Infectious Substances Safely
• Sample of a Customs Courtesy Letter
• Shipping Diagnostic and Clinical Samples Resources
• Transporting Infectious Substances by Ground
2. Disposal/Transfer/Lab Close-out Procedures
3. Equipment Decontamination Prior to Transfer or Disposal
4. Toxin Inactivation Report
5. Biohazard Label
6. Help Section
   • Sending Biological Samples to Foreign Countries
   • FedEx Guidelines: How to Pack Clinical, Biological Substance Category B, Limited Quantities, Dry Ice
      
16. Standard Microbiological Practices
1. Working with Biohazardous Materials
2. Biosafety Level 1 Sign
3. Biosafety Level 2 Sign
4. Methods of Containment
5. Glove Selection and Use
6. Personal Protective Measures
7. Biological Material Storage
    
17. Standard Operating Procedures, References/Tools
1. Writing Standard Operating Procedures (SOP)
2. Sample Biological Material Standard Operating Procedure Template
3. Aerosol Production and Exposure Control
4. Biosafety Level 2 Requirements
5. Centrifuge Safety
6. Methods of Containment
7. Examples of Biological Agents and Biosafety Level
8. MSDS
9. Risk Group Classification for Infectious Agents
10. Standard Operating Procedures for Flow Cytometry Cell Sorting
11. Guidance Document for University of Minnesota Microbiology Teaching Laboratories
     
18. Toxins of Biological Origin
    1. Biological Toxin Guidelines
    2. Toxin Fact Sheet
    3. NIH/CDC Guidelines for Work with Toxins of Biological Origin
    4. Inactivation of Biologically-derived Toxins
    5. Toxin Inactivation Report
    6. Examples of Biologically-derived Toxins
    7. IBC Biological Toxin Form
        
19. Training
    1. Research Lab Safety Training Requirements
    2. Shipper Training
    3. Bloodborne Pathogen Training Program
    4. Lab Safety Video Annotation Exercise: How Many Mistakes Can You Find?
       
    5. In-person training is available for groups of 15 or more
       • Biological and Infectious Waste
       • Biological Safety Cabinets
       • Decontamination
       • Working with Toxins of Biological Origin in the Laboratory (enrollment key = BIOTOXINS)
          
20. Waste Handling Procedures
1. Approved Disposal Methods for Infectious Agents
2. Autoclaving Biological Waste
3. Autoclave Testing Log
4. Waste Disposal Flow Chart
5. Infectious and Pathological Waste Management Plan
6. Chemotherapy Drug Disposal
7. Biological Waste Disposal Template
8. Inactivation of Biologically-derived Toxins
    

Reference:

1. CDC/NIH's Biosafety in Microbiological and Biomedical Laboratories (BMBL)
2. U of M Policy 2.1.13 Using Potentially Hazardous Biological Agent in Research and Teaching (2004)
   

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