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Biosafety Manual

The University of Minnesota's Biosafety Manual is made up of three components:

1. The following Biosafety Principles and Practices
2. CDC/NIH's Biosafety in Microbiological and Biomedical Laboratories (BMBL)
3. Individual lab-specific Standard Operating Procedures (SOPs)

University researchers must implement all three components in their lab safety manual.

1. Accidental Exposure/Emergency Response
1. General Emergency Procedures
2. Biohazards and Toxin Decontamination & Spill Clean-up
3. Biological Decontamination & Spill Clean-up Plan Template
4. Needlesticks and Other Infectious Exposure Procedures
5. Reporting Workers' Compensation Related Injuries
    
2. Autoclave Safety and Effectiveness
1. Autoclaving Biological Waste
2. Tips to Assure Effective Sterilization by Autoclave
3. Operator Maintenance
    
3. Biological Safety Cabinets
   1. Definition of Terms
   2. Types (Classes) of Biological Safety Cabinets
   3. Biological Safety Cabinet Placement
   4. Certification
   5. Use of Open Flames
   6. UV Lights
   7. Vacuum Lines
   8. Safe & Effective Work Practices
   9. Spill Cleanup
       
4. Bloodborne Pathogen/Human Infectious Agents
1. Bloodborne Pathogen Standard
2. Bloodborne Pathogen Training
3. University of Minnesota Exposure Control Plan
4. OSHA Bloodborne Pathogen Fact Sheets
5. Needlestick and Other Infectious Exposure Procedures
6. Minnesota Department of Health Slide Presentation for examples of safety engineered sharps see pages 14-19
7. 24 minute video: Workplace Precautions Against Bloodborne Pathogens
8. OSHA Bloodborne Pathogens and Needlestick Prevention
9. Biohazards and Toxin Decontamination & Spill Clean-up
10. Working with Human / Other Primate Cells, Tissues, or Human Derived Products
     
5. Decontamination and Disinfectants
1. Decontamination
2. Biological Decontamination & Spill Clean-up Plan Template
3. Biohazards and Toxin Decontamination & Spill Clean-up
4. Preparing Equipment for Service, Transfer, or Disposal
    
6. Fact Sheets: Safe Laboratory Practices
    
7. Prions
1. Scrapie Prion Research Procedures
2. U of M Requirements for Scrapie Prion Research
    
8. Recombinant DNA Procedures
    
9. Risk Assessment
1. Biological Risk Assessment in BMBL 5th edition
2. Risk Assessment Process
3. Self Assessment Form
4. Introduction to Risk Assessment
    
10. Select Agents
     
11. Sharps Usage
1. Sharps Safety
2. Sharps Safety Update
3. Minnesota Department of Health Slide Presentation for examples of safety engineered sharps see pages 14-19
    
12. Shipping, Transfer, or Disposal of Biological Materials
1. Shipping
• Guidelines for Shipping/Receiving Biological Materials
• Infectious Substance Classification Flow Chart
• Checklist for Diagnostic and Clinical Specimens
• Training:Shipping Hazardous and Infectious Materials
• Register Online for Shipping Hazardous and Infectious Materials Training Class
• Hazardous Material Shipping Program
• Shipping Exempt Patient Specimens
• Shipping Genetically Modified Organisms (GMOs)
• Shipper's Declaration of Dangerous Goods
• DOT training: Transporting Infectious Substances Safely
• Sample of a Customs Courtesy Letter
2. Disposal/Transfer/Lab Close-out Procedures
3. Equipment Decontamination Prior to Transfer or Disposal
4. Toxin Inactivation Report
5. Biohazard Label
    
13. Standard Microbiological Practices
1. Administrative Measures
2. Biohazard Sign
3. Methods of Containment
4. Glove Selection and Use
5. Personal Protective Measures
6. Biological Material Storage
    
14. Standard Operating Procedures, References/Tools
1. Writing Standard Operating Procedures (SOP)
2. Aerosol Production and Exposure Control
3. Biosafety Level 2 Requirements
4. Centrifuge Safety
5. Methods of Containment
6. Examples of Biological Agents and Biosafety Level
7. MSDS
8. Risk Group Classification for Infectious Agents
    
15. Toxins of Biological Origin
    1. NIH/CDC Guidelines for Work with Toxins of Biological Origin
    2. Inactivation of Biologically-derived Toxins
    3. Toxin Inactivation Report
    4. Examples of Biologically-derived Toxins
    5. Toxin Fact Sheet
        
16. Training
     
17. Waste Handling Procedures
1. Approved Disposal Methods for Infectious Agents
2. Autoclaving Waste
3. Waste Disposal Flow Chart
4. Infectious and Pathological Waste Management Plan
5. Chemotherapy Drug Disposal
6. Biological Waste Disposal Plan
7. Inactivation of Biologically-derived Toxins